Customs House is a heritage-listed customs house at 427 Queen Street, Brisbane CBD, Brisbane, Queensland, Australia. It was designed by Charles H McLay and built from 1886 to 1889 at a cost of £38,346 by John Petrie & Son. It was originally used for the collection of customs duty and was opened in 1889, when Queensland was a British colony, replacing the original Customs House located at Petrie Bight. It was added to the Queensland Heritage Register on 7 February 2005.